CRYSTAL-CLEAR MEMORIES OF A BACTERIUM
Globus R., Qimron U. | Science. 2017 Sep 15;357(6356):1096-1097
EXTENDING THE HOST RANGE OF BACTERIOPHAGE PARTICLES FOR DNA TRANSDUCTION
Yosef I., Goren M.G., Globus R., Molshanski-Mor S., Qimron U. | Mol Cell. 2017 Jun 1;66(5):721-728.e3. | Cover page – Molecular Cell June 1st 2017
SENSITIZING PATHOGENS TO ANTIBIOTICS USING THE CRISPR-CAS SYSTEM
Goren M., Yosef I., Qimron U. | Drug Resist Updat. 2017 Jan;30:1-6
REPEAT SIZE DETERMINATION BY TWO MOLECULAR RULERS IN THE TYPE I-E CRISPR ARRAY
Goren M.G., Doron S., Globus R., Amitai G., Sorek R., Qimron U. | Cell Rep. 2016 Sep 13;16(11):2811-2818
NATURAL SELECTION UNDERLIES APPARENT STRESS-INDUCED MUTAGENESIS IN A BACTERIOPHAGE INFECTION MODEL
Yosef I., Edgar R., Levy A., Amitai G., Sorek R., Munitz A., Qimron U. | Nat Microbiol. 2016 Apr 18;1(6):16047
SPACER ADAPTATION BIASES EXPLAIN PREFERENCE FOR FOREIGN DNA IN CRISPR ADAPTATION
Levy A.*, Goren M.G.*, Yosef I., Auster O., Manor M., Amitai G., Edgar R., Qimron U.†, #, Sorek R.†, # *contributed equally; †joint supervision of work; #corresponding author | Nature, (article), 520(7548):505-510, 2015 | Highlighted in Nature Reviews Microbiology and in numerous press releases
MICROBIOLOGY NEWS AND VIEWS: HOW BACTERIA GET SPACERS FROM INVADERS
Yosef I. and Qimron U. | Nature, 519(7542):166-167, 2015
THE CRISPR-CAS SYSTEM
The CRISPR-Cas system prevents phage growth by producing short RNA molecules that guide a cleavage complex to specific nucleic acids. We study the mechanisms and molecular players in this fascinating defense system. We are particularly interested in the adaptation step in which new short molecules that encode the guides are acquired.
BACTERIOPHAGES AS GENETIC TOOLS
Phages and their hosts co-evolved for billions of years. We explore novel phage interactions with their hosts to identify novel targets for drug design as well as provide novel inhibitors for such targets. We also use phages as the natural targets of the CRISPR-Cas system for elucidating mechanisms and developing new technologies. We further design phage particles able to package and deliver DNA molecules to an extended range of bacterial hosts, for targeting various pathogens.
REVERSING ANTIBIOTIC RESISTANCE OF PATHOGENS
We develop genetic constructs that eliminate the transfer of drug resistance genes between pathogens. Employment of counter-selection markers and the CRISPR-Cas system against genetic elements carrying drug resistance cassettes are designed for decreasing the spread of multi-drug resistant pathogens.